glucose free dmem  (Procell Inc)

Journal: Scientific Reports

Article Title: Oxidative stress imbalance and cellular damage mediated by the ND4 G11778A mutation

doi: 10.1038/s41598-026-40061-0

Figure Lengend Snippet: Mutant ND4 led to an imbalance of oxidative stress. The 661 W cells cultured in galactose or high glucose DMEM were stably expressing exogenous ND4. The levels of ROS ( A , B ) were measured. Under galactose conditions, an increase in ROS was evident in 661 W cells. Furthermore, cells expressing exogenous m.11778G > A genes displayed exacerbated changes. ( P = 0.0212, P< 0.0001, n = 6 per group). Scale bar = 100 μm. Data is presented as means ± SEM, n = 4, one-way ANOVA. **** P < 0.001.

Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM, high glucose, BIOEXPLORER, Cat#B1101-001); DMEM glucose-free medium (Basalmedia, Cat#L160KJ); Fetal bovine serum (FBS, Capricorn, Cat#F12A); Penicillin and streptomycin (Gibco, Cat#15140122); Galactose (Sigma-Aldrich, Cat#G5388); Puromycin (Basalmedia, Cat#S250J0); Cell-counting kit 8 (CCK-8) (Biosharp, Cat#BS350A); ROS assay kit (Solarbio, Beijing, China, Cat#CA1410); Superoxide Dismutase Assay Kit with WST-8 (Beyotime, Cat#S0101S); Catalase (CAT); Assay Kit (Solarbio, Cat#BC0205); GSH and GSSG Assay Kit (Beyotime, Cat#S0053); One-step TUNEL Apoptosis Kit (Elabscience, Cat#E-CK-A322); DAPI (Beyotime, Cat#C1005); Hoechst 33,342 (Solarbio, Cat#C0030); Seahorse XFe24 microplates (Agilent, Cat#02340); Agilent Seahorse XF DMEM medium (Agilent, Cat#103015); Sodium pyruvate (Agilent, Cat#103578); Glutamine (Agilent, Cat#103579); D-glucose (Agilent, Cat#103577); BCA protein assay kit (Beyotime, Cat#P0010); Mitochondrial Extraction Kit ( solarbio, Cat: SM0020); Blue/Clear Native-PAGE Gel Quick Preparation Kit (Meilunbio, MA0470); Acetylcysteine ( MedChemExpress, Cat#HY-B0215 ).

Techniques: Mutagenesis, Cell Culture, Stable Transfection, Expressing

Journal: Scientific Reports

Article Title: Oxidative stress imbalance and cellular damage mediated by the ND4 G11778A mutation

doi: 10.1038/s41598-026-40061-0

Figure Lengend Snippet: Mutant ND4 led to a decrease of antioxidative stress. The 661 W cells cultured in galactose or high glucose DMEM were stably expressing exogenous ND4. The activity of the enzymes SOD ( P = 0.0079, P <0.0001, n = 6 per group) ( A ) and T-GSH ( P = 0.0044, n = 4 per group) ( B ), and the level of CAT ( P = 0.0442, n = 4 per group) ( C ) were measured. Under high-glucose conditions, the activity of SOD and T-GSH were significantly reduced in 661 W cells. Under galactose conditions, the activity of SOD was significantly reduced in 661 W cells. Furthermore, cells expressing exogenous m.11778G > A genes displayed exacerbated changes. Scale bar = 100 μm. Data is presented as box and whiskers, one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM, high glucose, BIOEXPLORER, Cat#B1101-001); DMEM glucose-free medium (Basalmedia, Cat#L160KJ); Fetal bovine serum (FBS, Capricorn, Cat#F12A); Penicillin and streptomycin (Gibco, Cat#15140122); Galactose (Sigma-Aldrich, Cat#G5388); Puromycin (Basalmedia, Cat#S250J0); Cell-counting kit 8 (CCK-8) (Biosharp, Cat#BS350A); ROS assay kit (Solarbio, Beijing, China, Cat#CA1410); Superoxide Dismutase Assay Kit with WST-8 (Beyotime, Cat#S0101S); Catalase (CAT); Assay Kit (Solarbio, Cat#BC0205); GSH and GSSG Assay Kit (Beyotime, Cat#S0053); One-step TUNEL Apoptosis Kit (Elabscience, Cat#E-CK-A322); DAPI (Beyotime, Cat#C1005); Hoechst 33,342 (Solarbio, Cat#C0030); Seahorse XFe24 microplates (Agilent, Cat#02340); Agilent Seahorse XF DMEM medium (Agilent, Cat#103015); Sodium pyruvate (Agilent, Cat#103578); Glutamine (Agilent, Cat#103579); D-glucose (Agilent, Cat#103577); BCA protein assay kit (Beyotime, Cat#P0010); Mitochondrial Extraction Kit ( solarbio, Cat: SM0020); Blue/Clear Native-PAGE Gel Quick Preparation Kit (Meilunbio, MA0470); Acetylcysteine ( MedChemExpress, Cat#HY-B0215 ).

Techniques: Mutagenesis, Cell Culture, Stable Transfection, Expressing, Activity Assay